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Full-Length Complementary DNA of Hepatitis C Virus Genome From
an Infectious Blood Sample
Fig. 3. Detecting and sequencing of
the 3' terminal sequence of HCV from donor and recipient plasma.
(A) Detection of the terminal extra sequence in positive and
negative strands of HCV RNA and comparison with the presence of 5'
UTR sequence. The presence of the 3' extra sequence was examined by
RT-nested PCR and electrophoresed on a 3% agarose gel as described
in Materials and Methods. The primers used in RT are indicated at
the left. Arrows indicate the expected size of RT-PCR products.
Lane 1, normal serum (N); lane 2, plasma sample of
the donor; lane 3, recipient sample 4 weeks posttransfusion
(4W);lane 4, 5 weeks posttransfusion (5W); lane 5, 14
weeks posttransfusion (14W); lane 6, 16 weeks
posttransfusion (16W); and lane 7, 1.5 years posttransfusion
(1.5Y). (B) Homology analysis of the 3' terminal sequence of HCV
derived from donor and recipient. The upper sequences represent the
3' terminal sequence of pNIHJ1. The lower sequences indicate the
terminal sequences obtained from the recipient 4 weeks
posttransfusion. The nucleotides in the 3' extra sequences are
numbered 1' to 98' above the sequences. The termination codon of
the ORF (TGA) and sequences of the primers used in PCR are
underlined.
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